How old school are you?
I had one of those Mondays where the usual high-tech solutions kept breaking. For example, the lovely $300 bag-sealer I use for western blots stopped sealing. Heating element died, or some such.
I hunted around for a while to find something the right shape and size so I could still get away with using less antibody...
Stupid problem to have, but antibody is expensive!
And don't even get me started on the computers. Tell me why it's easier to take pictures on a digital camera, then transfer them to a computer, then print them out, then walk to the printer?
HOW is that easier than just using film and then walking away, film in hand, all finished in one step??
I'm one of those people, I'll run columns with gravity on the benchtop, because I don't have one of those fancy-schmancy FPLC machines. Takes longer, sure.
If postdoc time is 'free', tell that to my gray hair.
Even when I run a multiplex PCR, I still pipette them one at a time, by hand. One of these days I swear I'll get an undergrad to use a multipipettor and do them in octuplicate, or whatever... but not yet.
How old school are you? I'm looking for ridiculous but kick-ass, and preferably ghetto solutions to everyday lab problems.
Do you boil your protein samples with a bunsen burner and a marshmallow fork?
Do you seal your agarose gel trays with lab tape?
Do you clamp your glass plates with big rusty binder clips? Are they caked with years of buffer drippings? Hmmm? Are they????
And please, no mouth-pipetting stories. That's just gross.